CSEC BIOLOGY · LAB PREPARATION · LESSON L10

Compulsory Practical 5: Germination

Investigating the conditions needed for germination, measuring seedling growth, and drawing a dicot seed.

Practical Skills and the SBAB8.1–8.3SBA topic 5≈ 25 min🎮 4 games
Before you start

By the end of this lesson you can…

Step 1 · Watch

Explainer video

3 narrated scenes. Press play, listen and read along — the points appear as they are explained. Use ‹ Prev / Next › or the scene bar to jump to any scene.

LESSON L10 · EXPLAINER

Compulsory Practical 5: Germination

Four test tubes, one variable each. Germination is the perfect first controlled experiment.

Narration uses your device’s built-in voice. No sound? Switch to “Captions only” — the captions advance on their own.

Step 2 · Read

Key notes

Copy these into your notebook in your own words — that is how they stick.

Conditions

Water, oxygen, suitable temperature (light not needed for most seeds).

Removing oxygen

Boiled cooled water + oil layer (or pyrogallol in an advanced set-up).

Growth measures

Length, leaf number, dry mass; average several seeds.

Seed drawing

Testa, micropyle, hilum, cotyledons, plumule, radicle; title + magnification.

EXAM TIPS & COMMON MISTAKES

  • Change only ONE condition per tube and say which tube is the control.
Step 2 · Learn from examples

Worked examples & study resources

The kind of support a teacher gives after the lesson: worked examples to attempt first, a board illustration, key terms, common misconceptions and memory aids.

Worked example 1

Percentage germination

Of 25 seeds kept at 25 °C, 22 germinated; of 25 kept at 10 °C, 6 germinated. Calculate the percentage germination for each and explain the difference.

Try it in your notebook first, then reveal the solution one step at a time.

  1. Percentage germination = seeds germinated ÷ seeds planted × 100.
  2. 25 °C: 22 ÷ 25 × 100 = 88%.
  3. 10 °C: 6 ÷ 25 × 100 = 24%.
  4. Explain: germination depends on enzymes breaking down stored food. At 10 °C enzymes work slowly, so fewer seeds germinated in the time; 25 °C is closer to their optimum.
Answer. 88% at 25 °C; 24% at 10 °C. Low temperature slows the enzymes needed for germination.
Examiner’s tip. Using percentages lets you compare batches with different numbers of seeds.
Worked example 2

Growth rate of a radicle

Day12345
Radicle length (mm)26132230
Calculate (a) the mean growth rate over days 1–5 and (b) the growth rate between days 3 and 4.

Try it in your notebook first, then reveal the solution one step at a time.

  1. (a) Mean rate = change in length ÷ time = (30 − 2) ÷ (5 − 1) = 28 ÷ 4 = 7 mm per day.
  2. (b) Days 3–4: (22 − 13) ÷ 1 = 9 mm per day.
  3. The rate is not constant: growth speeds up as the seedling establishes itself.
Answer. (a) 7 mm per day. (b) 9 mm per day.
Examiner’s tip. Rate = change ÷ time, with units (mm per day). State which time interval you used.
On the board · Germination practical: one condition missing per tube
TubeWaterOxygenWarmthHow the condition is removed
Control✓✓✓—
No water✗✓✓Dry cotton wool
No oxygen✓✗✓Boiled, cooled water under oil, or alkaline pyrogallol in a sealed tube
No warmth✓✓✗Refrigerator at about 4 °C

Each tube removes ONE condition; the control has all three.

Key terms

Percentage germination
Number of seeds germinated ÷ number planted × 100.
Radicle
Embryo root — the first part to emerge.
Growth rate
Change in size ÷ time taken.
Germination
The start of growth of a seed, shown by the radicle emerging.

Common misconceptions

  • ✗ Seeds in the dark will not germinate.✓ Most seeds germinate in the dark; light is needed later for photosynthesis.
  • ✗ Growth rate is the final length ÷ number of days.✓ Rate is the CHANGE in length ÷ time, starting from the first measurement.
  • ✗ A few seeds are enough.✓ Use many seeds (e.g. 20–25 per condition) so percentages are reliable.

Memory aid

Rate = change ÷ time — always with units, e.g. mm per day.

Real-life link

Seed companies test the germination percentage of each batch and print it on the packet, so farmers know how many seeds to sow per bed.

Try this

Soak 20 bean seeds and keep 10 in the fridge and 10 at room temperature on damp paper. Count how many germinate after five days and calculate percentage germination.

Play & practise

Games

4 quick games on this lesson. Earn XP and badges as you play — your best score for each game is saved. Games are for practice and do not change your exam readiness.

Step 3 · Practise

Paper 1 practice — multiple choice

6 items modelled on real CSEC Paper 1 questions. Choose one answer for each, then check. Aim for 80% or better.

EduTech lab item

1. Why is the water boiled and covered with oil in one tube?

Answer: B. Tests the need for oxygen.
EduTech lab item

2. Which tube is the control?

Answer: B. All conditions present.
Adapted from CSEC Specimen Paper 01, Item 40

3. Which is associated with germination?

Answer: A. Seeds absorb water first.
Adapted from CSEC Specimen Paper 01, Item 41

4. Which is NOT used to measure growth in plants?

Answer: A. Volume is impractical.
Adapted from CSEC Specimen Paper 01, Item 42

5. A seed develops from the

Answer: C. Ovule → seed.
EduTech lab item

6. Mean shoot lengths of 12, 14 and 16 mm give a mean of

Answer: D. 42 ÷ 3.
Step 4 · Apply

Practical-style questions

Write full answers, then reveal the mark scheme and tick the points you earned (7 marks in total). Your answers are saved on this device.

Based on SO B8.1–8.3 and SBA topic 5

Question 1 [7 marks]

A student investigates the conditions needed for germination of bean seeds.

(a) Describe four tubes that test water, oxygen and temperature, identifying the control. [4]

MARK SCHEME — (a) (max 4)

(b) State the expected results and explain why seeds need water. [3]

MARK SCHEME — (b) (max 3)

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Sources

Syllabus sources and past papers

Everything in this lesson is based on these parts of the CXC syllabus and papers.