CSEC BIOLOGY · LAB PREPARATION · LESSON L8

Compulsory Practical 3: Photosynthesis and Respiration

Destarching, the leaf starch test, testing whether light, chlorophyll and CO₂ are needed, and showing respiration with limewater and yeast.

Practical Skills and the SBAB2.2–2.4B3.1–3.2SBA topic 3≈ 30 min🎮 3 games
Before you start

By the end of this lesson you can…

Step 1 · Watch

Explainer video

4 narrated scenes. Press play, listen and read along — the points appear as they are explained. Use ‹ Prev / Next › or the scene bar to jump to any scene.

LESSON L8 · EXPLAINER

Compulsory Practical 3: Photosynthesis and Respiration

To prove a plant needs carbon dioxide you must remove it — and keep everything else the same. That’s experimental design.

Narration uses your device’s built-in voice. No sound? Switch to “Captions only” — the captions advance on their own.

Step 2 · Read

Key notes

Copy these into your notebook in your own words — that is how they stick.

Destarching

Plant in darkness 24–48 h so starch is used up.

Starch test

Boil in water → ethanol in water bath (Bunsen off) → rinse → iodine (blue-black = starch).

Light

Foil-covered leaf part stays brown.

Chlorophyll

Variegated leaf: only green parts make starch.

CO₂

KOH (or soda lime) absorbs CO₂; control uses water.

Respiration

Limewater turns milky with CO₂; boiled seeds as control; thermos shows heat.

EXAM TIPS & COMMON MISTAKES

  • In each experiment, name the ONE factor removed and the control.
Step 2 · Learn from examples

Worked examples & study resources

The kind of support a teacher gives after the lesson: worked examples to attempt first, a board illustration, key terms, common misconceptions and memory aids.

Worked example 1

A variegated leaf with a foil strip

A destarched variegated leaf (green with white edges) had a strip of foil placed across it. After 6 hours in sunlight it was tested with iodine. Predict the colour of (a) green parts not covered, (b) green parts under the foil, (c) white parts. Explain.

Try it in your notebook first, then reveal the solution one step at a time.

  1. (a) Green, uncovered: blue-black — chlorophyll and light were both present, so photosynthesis made starch.
  2. (b) Green, under foil: yellow-brown — no light, so no photosynthesis and no starch.
  3. (c) White parts: yellow-brown — no chlorophyll, so no photosynthesis even in light.
  4. Destarching first (plant kept in the dark for 48 hours) ensures any starch found was made during the experiment.
Answer. (a) Blue-black. (b) Yellow-brown. (c) Yellow-brown. Starch is made only where both light and chlorophyll are present.
Examiner’s tip. Explain WHY you destarch: so that any starch detected was made during the experiment, not before it.
Worked example 2

Hydrogencarbonate indicator tubes

Hydrogencarbonate indicator is red/orange at normal CO₂ levels, yellow when CO₂ rises and purple when CO₂ falls. Four tubes were set up for 3 hours: A — pondweed in light; B — pondweed in the dark; C — snail in light; D — indicator only, in light. Predict and explain the colours.

Try it in your notebook first, then reveal the solution one step at a time.

  1. A (plant, light): photosynthesis is faster than respiration, so CO₂ is removed → purple.
  2. B (plant, dark): only respiration, adding CO₂ → yellow.
  3. C (snail, light): animals only respire, adding CO₂ → yellow.
  4. D: no organism → stays red/orange. This is the control, showing that light alone does not change the colour.
Answer. A purple (net CO₂ uptake by photosynthesis); B yellow (respiration only); C yellow (respiration); D red/orange (control).
Examiner’s tip. Plants respire in the light too — tube A turns purple only because photosynthesis is FASTER than respiration.
On the board · The starch test on a leaf — every step has a reason
StepReason
Boil the leaf in water for about 1 minuteKills the cells, stops reactions, makes the cells permeable to iodine
Turn off the Bunsen; put the leaf in ethanol in a hot water bathRemoves chlorophyll so colour changes can be seen (ethanol is flammable)
Dip in warm waterSoftens the leaf, which ethanol made brittle
Spread on a white tile; add iodineBlue-black = starch present; yellow-brown = no starch

Learn the reason with each step; most marks are for the reasons.

Key terms

Destarching
Keeping a plant in the dark for 48 hours so it uses up its stored starch.
Variegated leaf
A leaf with green (chlorophyll) and white (no chlorophyll) areas.
Hydrogencarbonate indicator
Shows CO₂ level: yellow (high), red/orange (normal), purple (low).
Soda lime / KOH
Absorbs carbon dioxide from the air around a leaf or organism.
Limewater
Turns milky (cloudy) when carbon dioxide is bubbled through it.

Common misconceptions

  • ✗ The ethanol is heated directly over the Bunsen.✓ Ethanol is flammable — heat it in a water bath with the flame off.
  • ✗ Green plants do not respire in light.✓ They respire all the time; in light, photosynthesis is faster.
  • ✗ A yellow-brown result means the test failed.✓ Yellow-brown is the negative result — it shows no starch was made.

Memory aid

Leaf starch test: Boil, Bleach (ethanol), Bathe (warm water), Brown-or-Black (iodine).

Real-life link

Greenhouse growers sometimes raise CO₂ levels and add lighting because they know light, CO₂ and chlorophyll are all needed for the plant to make food.

Try this

Cover part of a leaf on a potted plant with foil for two days. Ask your teacher to help you test it for starch and compare the covered and uncovered parts.

Play & practise

Games

3 quick games on this lesson. Earn XP and badges as you play — your best score for each game is saved. Games are for practice and do not change your exam readiness.

Step 3 · Practise

Paper 1 practice — multiple choice

6 items modelled on real CSEC Paper 1 questions. Choose one answer for each, then check. Aim for 80% or better.

Adapted from CSEC Specimen Paper 01, Item 15

1. Plants are kept in the dark before a starch experiment to

Answer: B. Destarching ensures starch found was made during the test.
Adapted from CSEC Specimen Paper 01, Item 14

2. In the bell-jar experiment, potassium hydroxide is used to

Answer: C. It removes CO₂ from the air in the jar.
Adapted from CSEC Specimen Paper 01, Item 14

3. The aim of the bell-jar experiment is to find out

Answer: A. Only CO₂ differs.
EduTech lab item

4. Ethanol is used in the starch test to

Answer: D. Chlorophyll would hide the colour change.
EduTech lab item

5. Limewater turns milky in the presence of

Answer: A. Test for CO₂.
EduTech lab item

6. A suitable control for germinating seeds producing CO₂ is

Answer: B. Dead seeds do not respire.
Step 4 · Apply

Practical-style questions

Write full answers, then reveal the mark scheme and tick the points you earned (8 marks in total). Your answers are saved on this device.

Based on CSEC Specimen Paper 01 Items 14–15 and P2 May/June 2021 Q1(f)

Question 1 [8 marks]

A student wants to show that carbon dioxide is needed for photosynthesis.

(a) Describe the set-up, including the control. [4]

MARK SCHEME — (a) (max 4)

(b) Describe how a leaf is tested for starch and give the expected results. [4]

MARK SCHEME — (b) (max 4)

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Sources

Syllabus sources and past papers

Everything in this lesson is based on these parts of the CXC syllabus and papers.