CSEC BIOLOGY · SECTION B · LESSON B10

Transport in Plants: Xylem, Transpiration and Phloem

How water travels from soil to leaf, why transpiration matters, the factors that change its rate, water conservation, and translocation in phloem.

Life Processes and DiseaseSO B 4.7SO B 4.8SO B 4.9SO B 4.10SO B 4.11≈ 35 min🎮 4 games
Before you start

By the end of this lesson you can…

Step 1 · Watch

Explainer video

6 narrated scenes. Press play, listen and read along — the points appear as they are explained. Use ‹ Prev / Next › or the scene bar to jump to any scene.

LESSON B10 · EXPLAINER

Transport in Plants: Xylem, Transpiration and Phloem

A mango tree lifts water 15 m high with no pump and no heart. How?

Narration uses your device’s built-in voice. No sound? Switch to “Captions only” — the captions advance on their own.

Explore in 3D

The stoma in 3D

See how guard cells open and close the pore.

Step 2 · Read

Key notes

Copy these into your notebook in your own words — that is how they stick.

Xylem

Dead, hollow, lignified vessels with no end walls → continuous water column; strength & support.

Water uptake

Root hairs by osmosis → cortex → xylem → leaf mesophyll → evaporates → stomata.

Transpiration

Loss of water vapour from aerial parts, mainly via stomata.

Importance

Transpiration pull moves water and minerals; cooling; turgidity; water for photosynthesis.

Factors

Temperature ↑, wind ↑, light ↑ → rate ↑; humidity ↑ → rate ↓.

Water conservation

Thick cuticle, sunken/few stomata, spines, rolled leaves, hairs, succulence, deep roots, leaf fall.

Phloem

Living sieve tubes with sieve plates + companion cells (energy). Translocation of sucrose from source to sink.

Source

Where sucrose is loaded into phloem (e.g. photosynthesising leaf).

EXAM TIPS & COMMON MISTAKES

  • For each transpiration factor, state the EFFECT and the REASON — usually 2 marks each.
  • The P1 “highest rate” answer: sunny AND high wind speed.
  • Phloem has few organelles yet transports food because companion cells supply the energy (ATP) for loading.
Step 2 · Learn from examples

Worked examples & study resources

The kind of support a teacher gives after the lesson: worked examples to attempt first, a board illustration, key terms, common misconceptions and memory aids.

Worked example 1

Using a potometer

In a potometer, an air bubble moved 45 mm in 5 minutes in still air, and 90 mm in 5 minutes when a fan blew on the shoot. (a) Calculate the rate in each case in mm per minute. (b) Explain the difference. (c) Why does a potometer not measure transpiration exactly?

Try it in your notebook first, then reveal the solution one step at a time.

  1. (a) Still air: 45 ÷ 5 = 9 mm per minute. With the fan: 90 ÷ 5 = 18 mm per minute.
  2. (b) Moving air carries away the water vapour that builds up around the leaves, keeping a steep concentration gradient between the air spaces in the leaf and the air outside, so water vapour diffuses out of the stomata faster. More water is drawn up the xylem to replace it.
  3. (c) A potometer measures water uptake. A small amount of the water taken up is used in photosynthesis or kept in cells, so uptake is slightly more than the water lost by transpiration.
Answer. (a) 9 mm/min and 18 mm/min — the rate doubled. (b) Wind removes humid air from around the leaf, keeping a steep diffusion gradient. (c) It measures water uptake, not water loss.
Examiner’s tip. To make a fair comparison, keep the same shoot and the same light, temperature and humidity, and change only the air movement.
Worked example 2

Where are the stomata?

Four similar leaves were treated with petroleum jelly (which blocks stomata) and hung up for 3 days.
LeafTreatmentMass lost (g)
ANo jelly3.0
BJelly on upper surface2.6
CJelly on lower surface0.6
DJelly on both surfaces0.1
What do the results show? Explain them.

Try it in your notebook first, then reveal the solution one step at a time.

  1. Mass is lost mainly as water vapour through the stomata.
  2. Blocking the upper surface (B) reduced loss only a little (3.0 → 2.6 g), so few stomata are on the upper surface.
  3. Blocking the lower surface (C) reduced loss greatly (3.0 → 0.6 g), so most stomata are on the lower surface.
  4. D still lost 0.1 g — a little water evaporates through the waxy cuticle even when the stomata are blocked.
  5. Having most stomata underneath reduces water loss, because the lower surface is shaded and cooler.
Answer. Most water is lost from the lower surface, so most stomata are on the lower surface. A little water is lost through the cuticle (leaf D).
Examiner’s tip. Leaf A is the control. Always say what the control is for: it shows the water loss with no treatment, for comparison.
On the board · Xylem and phloem
XylemPhloem
CarriesWater and mineral ionsSucrose and amino acids (translocation)
DirectionUpwards only (roots → leaves)Both ways — from source (leaves) to sink (roots, fruits, growing tips)
CellsDead, hollow tubes with no end wallsLiving sieve tubes with sieve plates and companion cells
WallsThickened with lignin (also gives support)Thin cellulose walls
Driven byTranspiration pull from the leavesActive processes in living cells (energy needed)

Xylem: water and minerals, up only, dead cells. Phloem: sugars, both directions, living cells.

Key terms

Transpiration
Loss of water vapour from the leaves, mainly through the stomata.
Transpiration stream
Movement of water from roots, up the xylem, to the leaves.
Translocation
Movement of sugars and amino acids through the phloem.
Xylem
Tissue of dead, lignified vessels carrying water and minerals upwards.
Phloem
Tissue of living sieve tubes carrying food materials.
Potometer
Apparatus that measures the rate of water uptake by a shoot.
Wilting
Drooping of a plant when cells lose turgor because water loss exceeds uptake.

Common misconceptions

  • ✗ A potometer measures transpiration.✓ It measures water uptake, which is almost — but not exactly — the same as water loss.
  • ✗ Phloem carries food only downwards.✓ Phloem carries food from where it is made or stored (source) to where it is needed (sink), up or down.
  • ✗ Transpiration is just a waste of water.✓ It pulls water and minerals up the xylem, cools the leaf, and keeps cells turgid.

Memory aid

Xylem = X-tra water, going up. Phloem = food. Transpiration speeds up with Wind, Warmth, Bright light and Dry air.

Real-life link

Plants on dry Caribbean coasts and islands — such as cacti and the century plant (Agave) — conserve water with thick waxy cuticles, few or sunken stomata and swollen stems or leaves that store water.

Try this

Stand a stick of celery or a white flower in water coloured with food dye. After a day, cut across the stem and look for the coloured dots — those are the xylem vessels.

Play & practise

Games

4 quick games on this lesson. Earn XP and badges as you play — your best score for each game is saved. Games are for practice and do not change your exam readiness.

Step 3 · Practise

Paper 1 practice — multiple choice

8 items modelled on real CSEC Paper 1 questions. Choose one answer for each, then check. Aim for 80% or better.

Adapted from CSEC P1 May/June 2023 & 2024, Item 14

1. The major feature responsible for the rigid structure of the xylem vessel is

Answer: A. Lignin strengthens the walls.
Adapted from CSEC P1 2022 & 2024, Item 29

2. Under which conditions is transpiration HIGHEST?

Answer: A. Light opens stomata; wind keeps the gradient steep.
Adapted from CSEC P1 2022, 2023, 2024, Item 28/30

3. In phloem transport, a source is any area of the plant where

Answer: C. Leaves are sources; they load sucrose.
EduTech practice item (SO B4.9)

4. Increasing humidity around a plant will

Answer: C. Less concentration gradient between leaf and air.
Based on CSEC P2 May/June 2026, Q1(g)

5. Which adaptation of a cactus reduces water loss?

Answer: D. Spines have a very small surface area.
EduTech practice item

6. Water moves into root hair cells by

Answer: B. Soil water is more dilute than cell sap.
EduTech practice item

7. Companion cells help phloem sieve tubes by

Answer: B. Sieve tubes have few organelles; companion cells supply ATP.
EduTech practice item

8. Which apparatus is used to measure the rate of water uptake by a leafy shoot?

Answer: D. A potometer measures water uptake.
Step 4 · Apply

Paper 2 practice — structured questions

Write full answers, then reveal the mark scheme and tick the points you earned (23 marks in total). Your answers are saved on this device.

Based on CSEC P2 January 2024, Question 4(a)–(d)

Question 1 [12 marks]

Transpiration in flowering plants.

(a) Define the term “transpiration”. [1]

MARK SCHEME — (a) (max 1)

(b) State TWO reasons why transpiration is important to plants. [2]

MARK SCHEME — (b) (max 2)

(c) List THREE factors, other than temperature, that affect transpiration, and explain how each affects the rate. [9]

MARK SCHEME — (c) (max 9)

Based on CSEC P2 May/June 2023 Q4(b), May/June 2024 Q1(e), January 2026 Q1(d)

Question 2 [11 marks]

Xylem and phloem are specialised plant tissues.

(a) Define “translocation” and explain TWO ways xylem is suited to transpiration. [5]

MARK SCHEME — (a) (max 5)

(b) Explain why phloem cells, which have very few organelles, can still transport food to all parts of the plant. [2]

MARK SCHEME — (b) (max 2)

(c) Explain TWO ways in which plants conserve water. [4]

MARK SCHEME — (c) (max 4)

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Sources

Where this topic appears in CSEC papers

Find these in your own past-paper booklets for extra timed practice. The practice above is adapted from them.