CSEC BIOLOGY · SECTION A · LESSON A2

Carrying Out an Ecological Study

Quadrats, transects, capture–recapture and collecting equipment — how to choose and use the right sampling method.

Living Organisms in the EnvironmentSO A 2.1≈ 30 min🎮 3 games
Before you start

By the end of this lesson you can…

Step 1 · Watch

Explainer video

7 narrated scenes. Press play, listen and read along — the points appear as they are explained. Use ‹ Prev / Next › or the scene bar to jump to any scene.

LESSON A2 · EXPLAINER

Carrying Out an Ecological Study

How could you count every crab on a mangrove shore without catching them all? You sample.

Narration uses your device’s built-in voice. No sound? Switch to “Captions only” — the captions advance on their own.

Step 2 · Read

Key notes

Copy these into your notebook in your own words — that is how they stick.

Sample

A small representative part of a habitat studied to estimate the whole. Must be random and repeated.

Quadrat

Square frame (usually 1 m²) for plants and slow-moving animals. Measures density, frequency or % cover.

Transect

Line across a habitat along an environmental gradient. Line transect records touches; belt transect uses quadrats at intervals. Shows zonation.

Capture–recapture

N = (first sample × second sample) ÷ marked recaptured. For mobile animals.

Assumptions

Harmless marks; marked animals mix evenly; no births, deaths or migration; marks stay on.

Pitfall trap

Container sunk level with soil to catch ground-dwelling invertebrates. Check it often; add a cover against rain.

Sweep / plankton net

Nets for flying insects in vegetation / small organisms in water.

Tullgren funnel

Heat and light from a lamp drive small soil animals down into alcohol or a jar.

EXAM TIPS & COMMON MISTAKES

  • Show the formula AND substitution in capture–recapture — the method mark is often separate from the answer mark.
  • Match the method to the organism: “mushrooms in a garden” → quadrat, not a transect.
  • When listing precautions, be specific: “place quadrats at random” scores; “be careful” does not.
Step 2 · Learn from examples

Worked examples & study resources

The kind of support a teacher gives after the lesson: worked examples to attempt first, a board illustration, key terms, common misconceptions and memory aids.

Worked example 1

Estimating a population by capture–recapture

Students caught 40 snails in a garden, marked each shell with a dot of paint and released them. Two days later they caught 50 snails, of which 10 were marked. Estimate the snail population.

Try it in your notebook first, then reveal the solution one step at a time.

  1. Write the Lincoln index: population = (number marked in first sample × total in second sample) ÷ number marked in second sample.
  2. Substitute the values: (40 × 50) ÷ 10.
  3. 40 × 50 = 2000, and 2000 ÷ 10 = 200.
  4. State the answer with units (number of snails) and say it is an estimate.
Answer. Estimated population = (40 × 50) ÷ 10 = 200 snails.
Examiner’s tip. Always show the formula and the substitution — the working usually earns a mark even if you slip on the arithmetic. Be ready to state the assumptions: marks do not rub off or harm the snails, marked snails mix evenly with the rest, and no snails are born, die, arrive or leave between the two samples.
Worked example 2

Estimating plants with a quadrat

A field measures 50 m². A 1 m² quadrat was thrown randomly 10 times and the number of Mimosa pudica (shame-bush) plants was counted each time: 3, 5, 0, 2, 4, 6, 1, 3, 2, 4. Estimate the number of these plants in the field.

Try it in your notebook first, then reveal the solution one step at a time.

  1. Add the counts: 3 + 5 + 0 + 2 + 4 + 6 + 1 + 3 + 2 + 4 = 30 plants.
  2. Find the mean per quadrat: 30 ÷ 10 = 3 plants per m².
  3. Scale up to the whole field: 3 plants per m² × 50 m² = 150 plants.
Answer. Mean density = 3 plants per m², so the field has about 150 plants.
Examiner’s tip. Random placement avoids bias towards the “interesting” patches. More quadrats make the estimate more reliable — mention this when asked how to improve the investigation.
On the board · Choosing the right sampling method
Method / apparatusUsed to sampleKey precaution
QuadratPlants and slow-moving animals in an areaPlace randomly; use many quadrats
Line / belt transectChange in organisms along a gradient (shore, pond edge)Record at regular intervals along the line
Pitfall trapGround-crawling invertebratesRim level with the soil; cover against rain; check often
Sweep netInsects in long grassSame number of sweeps at each site
Plankton netTiny organisms in waterTow at the same speed and depth
Tullgren funnelSmall animals in leaf litter or soilDo not overheat the sample
Capture–recaptureMobile animals (snails, woodlice, fish)Marks must be harmless and permanent

Match the method to the organism: fixed or slow organisms → quadrats and transects; fast-moving animals → traps and capture–recapture.

Key terms

Sample
A small part studied to represent the whole population or area.
Quadrat
A square frame of known area used to sample organisms.
Transect
A line across a habitat along which organisms are recorded.
Population density
Number of individuals per unit area, e.g. plants per m².
Percentage cover
The percentage of a quadrat’s area covered by one species.
Random sampling
Choosing sample positions by chance to avoid bias.
Lincoln index
Population = (first catch × second catch) ÷ marked recaptured.

Common misconceptions

  • ✗ One quadrat is enough if it is placed carefully.✓ One quadrat is not representative. Use many, randomly placed, and take the mean.
  • ✗ Capture–recapture can be used for plants.✓ It is used for mobile animals. Plants are counted directly with quadrats.
  • ✗ Any mark will do.✓ Marks must not harm the animal or make it easier for predators to see, and must not rub off before the second sample.

Memory aid

Lincoln index: “First times Second, over Marked-in-second” — F × S ÷ M.

Real-life link

Fisheries officers across the Caribbean use sampling and mark–recapture data to estimate stocks of fish and spiny lobster, and use those estimates to set closed seasons and catch limits.

Try this

Make a 0.5 m × 0.5 m quadrat from string and four sticks. Throw it ten times on a lawn, count one type of plant in each throw, and estimate how many there are on the whole lawn.

Play & practise

Games

3 quick games on this lesson. Earn XP and badges as you play — your best score for each game is saved. Games are for practice and do not change your exam readiness.

Step 3 · Practise

Paper 1 practice — multiple choice

8 items modelled on real CSEC Paper 1 questions. Choose one answer for each, then check. Aim for 80% or better.

Adapted from CSEC P1 May/June 2022, Item 2

1. Which sampling method is MOST suitable for estimating the density of mushrooms growing on a lawn?

Answer: D. Mushrooms do not move and are spread over an area — random quadrats give density.
EduTech practice item (SO A2.1)

2. In a study, 30 snails were marked and released. Later 40 were caught, of which 8 were marked. The estimated population is

Answer: C. (30 × 40) ÷ 8 = 150.
EduTech practice item

3. A belt transect is used rather than random quadrats when

Answer: C. Transects show how organisms change along an environmental gradient (zonation).
EduTech practice item

4. Which piece of apparatus is used to collect small insects from a leaf without harming them?

Answer: B. A pooter sucks the insect into a container.
Based on CSEC P2 May/June 2026, Q3(c)

5. Which is NOT an assumption of the capture–recapture method?

Answer: A. The method assumes a stable, closed population — not a rapidly changing one.
EduTech practice item

6. Why are quadrats placed at random positions?

Answer: D. Random placement prevents the student choosing “interesting” spots.
EduTech practice item

7. A pitfall trap would be BEST for sampling

Answer: B. Pitfall traps catch animals that crawl along the ground.
EduTech practice item

8. Percentage cover is MOST useful for recording

Answer: A. When individuals cannot be separated, estimate the % of the quadrat they cover.
Step 4 · Apply

Paper 2 practice — structured questions

Write full answers, then reveal the mark scheme and tick the points you earned (14 marks in total). Your answers are saved on this device.

Based on CSEC P2 May/June 2026, Question 3(a)–(c)

Question 1 [7 marks]

Jacob found many wood lice near his home. He caught 20, marked each with a small dot of paint and released them. One week later he caught 50 wood lice, of which 10 were marked.

(a) Apart from capture–recapture, list THREE methods used by ecologists to study organisms in an ecosystem. [3]

MARK SCHEME — (a) (max 3)

(b) Using a suitable equation, estimate the number of wood lice in the area. [2]

MARK SCHEME — (b) (max 2)

(c) Suggest TWO assumptions Jacob made when using this method. [2]

MARK SCHEME — (c) (max 2)

Based on SO A2.1 practical (SBA ecological study)

Question 2 [7 marks]

A class wants to compare the plants growing near a pond with those on a dry hillside 30 m away.

(a) Name a suitable sampling method and describe how it would be carried out. [4]

MARK SCHEME — (a) (max 4)

(b) State TWO precautions the students should take. [2]

MARK SCHEME — (b) (max 2)

(c) Suggest ONE abiotic factor that could explain the differences in plants. [1]

MARK SCHEME — (c) (max 1)

Your progress

Lesson readiness

0%
READY
Not started
Video watched (20%)0%
Multiple choice (40%)0%
Structured (40%)0%

Sources

Where this topic appears in CSEC papers

Find these in your own past-paper booklets for extra timed practice. The practice above is adapted from them.